Structural Insights into the Processing of Nucleobase-Modified Nucleotides by DNA Polymerases

Asymmetric Recognition of Nucleobase Features by DNA Polymerases

Structural basis for the synthesis of nucleobase modified DNA by Thermus aquaticus DNA polymerase.

Numerous 2'-deoxynucleoside triphosphates (dNTPs) that are functionalized with spacious modifications such as dyes and affinity tags like biotin are substrates for DNA polymerases. They are widely employed in many cutting-edge technologies like advanced DNA sequencing approaches, microarrays, and single molecule techniques. Modifications attached to the nucleobase are accepted by many DNA polym...

Incorporation of reporter-labeled nucleotides by DNA polymerases.

The incorporation of fluorescently labeled nucleotides into DNA by DNA polymerases has been used extensively for tagging genes and for labeling DNA. However, we lack studies comparing polymerase efficiencies for incorporating different fluorescently labeled nucleotides. We analyzed the incorporation of fluorescent deoxynucleoside triphosphates by 10 different DNA polymerases, representing a cro...

Recognition of threosyl nucleotides by DNA and RNA polymerases.

Alpha-L-threose nucleic acids (TNA) are potentially natural nucleic acids that could have acted as an evolutionary alternative to RNA. We determined whether DNA or RNA polymerases could recognize phosphorylated threosyl nucleosides. We found that for both the Vent (exo-) DNA polymerase and HIV reverse transcriptase K(m) values were increased and kcat values decreased for the incorporation of tT...

New insights into selectivity of DNA polymerases: A combinatorial approach

" Mutierte DNA-Polymerasen mit erhöhter Fehlpaarungsdiskriminierung " Based on family A DNA polymerases Patent in preparation N. Z. Rudinger, A. Marx " Mutierte DNA-Polymerasen mit erhöhter Fehlpaarungsdiskriminierung " Based on family B DNA polymerases 1 Introduction The propagation of genetic information from one generation to the next is a central part of evolution. In this context, replicat...